To investigate the mechanism involved in the treatment of PAP on CUMS-induced depression, the protein expression levels of p-AMPK, Sirt1, Ac-NF-κB, NF-κB, NLRP3, ASC, Ac-Caspase-1, GSDMD-N, Cleaved-IL-1β, and Cleaved-IL-18 were analyzed by Western blot (Figure 3). This evidence concerns the gene IL18 and major depressive disorder.