Although these results do not rule out the possibility that C5b may also inhibit ASIC2 channels at less acidic conditions, the dependence of the ASIC2 channels on the extremely low pH makes them unlikely targets of C5b during tissue acidosis associated with cerebral ischemia, which has a pH range of 6.0–6.7 (Huang et al., 2015; Cui et al., 2016). Here, ASIC2 is linked to Cerebral ischemia.