Figures 2B–E show the effect of CRCR on the pharmacological indexes. Significant reduction of serotonin [5-hydroxytryptamine (5-HT)] and significant improvement of calcitonin gene-related peptide (CGRP), nitric oxide (NO), and nitric oxide synthase (NOS) were observed in the migraine rats compared with the control rats (p < 0.0001, p < 0.01, p < 0.0001, p < 0.0001). With the treatment of CRCR, 5-HT was significantly increased (p < 0.0001), CGRP and NOS were significantly decreased (p < 0.0001, p < 0.05), which indicated CRCR had a good antimigraine effect on NTG-induced migraine rats. This evidence concerns the gene OPA1 and migraine disorder.