The KEGG pathway enrichment analysis showed that the treatment of IBS with Sini Powder and Tong xie yao fang decoction involves multiple signaling pathways, such as cancer, neuroactive ligand-receptor interaction, calcium ion signaling, cAMP, HIF-1, and hepatitis B. The cAMP signaling pathway maintains the stability of the internal and external environment of intestinal cells, improves enteric nerve function, and regulates intestinal secretion and absorption [33]. This evidence concerns the gene HIF1A and irritable bowel syndrome.