Our results showed that the overall proportion of CD3+ CD4+ T and CD3+ CD8+ T cells in tissues was reduced, and tumor-infiltrating T lymphocytes (TIL CD4+ T, TIL CD8+ T) were significantly reduced in Tmem176b−/−mice, which is consistent with our findings from public databases, where the higher the expression of TMEM176B, the higher the degree of T cell infiltration in SKCM. The gene discussed is CD4; the disease is neoplasm.