We analyzed the expression of TMEM176B in SKCM with the expression of some T cell-related genes to confirm our findings in the mouse tumor model, and we discovered that the expression of TMEM176B was significantly and positively linked with the expression of immunosuppressive molecules CD274 (PD-L1), CTLA4, LAG3, and cytotoxic molecules associated with T cell function TNF, PRF1, GZMA, GZMK, and GZMM, and most cytotoxic molecules, have been validated to act mainly as anti-tumoral and anti-infectious factors (Arias et al., 2017). Here, PRF1 is linked to neoplasm.