By comparing the relative expression levels of cell markers (HCs: Alb, HSCs: α-SMA, GFAP, Col1α1 and Col1α3; KCs: F4/80), the results showed that Alb was predominantly expressed in HCs, with the percentage of 69.44, F4/80 predominantly expressed in KC with 73.19, and α-SMA, GFAP, Col1α1 and Col1α3 predominantly expressed in HSC (Supplementary Figure S1) with 83.09, 87.70, 93.36 and 89.55, respectively, suggesting each type of cell was enrichment. Here, ACTA1 is linked to keratoconus.