In addition, KEGG pathway analyses indicated that target genes of let-7a-5p were significantly enriched in signaling pathways of PI3K-Akt, MAPK and AMPK, and in ECM-receptor interaction, focal adhesion, amoebiasis, which are all involved in the process of mechanotransduction (Supplementary Figure S8B). This evidence concerns the gene AKT1 and amebiasis.