SNCA and Parkinson disease: We used an established SH-SY5Y cell-line which stably expresses human wild-type α-syn N-terminally fused to enhanced green fluorescent protein (EGFP) (SH-SY5YWT) as previously described (49), thus mimicking autosomal dominant forms of PD caused by multiplication of the α-syn-encoding SNCA locus (37,38).