LRATD2 and esophageal squamous cell carcinoma: To elucidate the biological effect of FAM84B copy number amplification in ESCC, the protein levels of FAM84B in ESCC cell lines were tested, including the immortal embryonic esophageal epithelium cell lines NE-2, and ESCC cell lines KYSE180, KYSE150, KYSE450 by western blot (Fig. S3A).