Signature RS1 (47%) was associated with ecDNA events encompassing (log odds 3.28, p-value = 0.004, logistic regression, Supplementary Data 4) and increased expression of CCNE1 compared to other tumours (log odds 0.88, p-value = 1.55 × 10−4, logistic regression, Supplementary Data 4, p = 7.5 × 10-7, Wilcoxon rank sum test, Supplementary Fig. S2J). The gene discussed is CCNE1; the disease is neoplasm.