When fluorescently labeled primary human peripheral blood mononuclear cells (PBMCs) were flowed through the endothelium-lined vascular channel 24 hpi with influenza H3N2, we detected upregulation of ICAM1 and TNF in the endothelial cells by qPCR, indicating endothelial inflammation (Supplementary Fig. 6d). The gene discussed is TNF; the disease is influenza.