To determine whether RAGE and TRPV4 mediate the effects of mechanical strain on innate immunity, we perfused the RAGE inhibitor azeliragon or the TRPV4 inhibitor GSK2193874 at non-toxic doses (Supplementary Fig. 11) through the vascular channel of the alveolus chip for 48 hours before infection with H3N2 while applying 5% mechanical strain (Fig. 7a). The gene discussed is TRPV4; the disease is infection.