To determine the relevance of these residues for the STUB1-mediated regulation of IFNγ-R1 and JAK1, we generated melanoma cell clones deficient in both IFNGR1 and JAK1 (IFNGR1-KO + JAK1-KO) in either a wildtype or STUB1-deficient background. This evidence concerns the gene JAK1 and melanoma.