To further elucidate the role of MSC-ex derived BECN1 in ferroptosis induction and liver fibrosis amelioration, we compared the role of MSC-ex with shRNA control (Exsh-ctr) or BECN1 knockdown (ExshBECN1) in the regulation of GPX4 and a-SMA expression, ROS production, and hepatic collagen deposition in vitro and in vivo. The gene discussed is BECN1; the disease is Hepatic fibrosis.