NANOG and non-small cell lung carcinoma: Ninj1high subpopulations in H460 and A549 cells exhibited increased CSC properties, including CSC-associated marker gene expression (i.e., ALDH1A1, POU5F1, NANOG, and SOX2) (Fig. 2j), tumorsphere formation [8] (Fig. 2k), and tumorigenicity in the limiting dilution assay (P = 0.0171) (Fig. 2l) compared to these characteristics the Ninj1low subpopulations and their corresponding NSCLC cells.