Next, we performed cell proliferation, invasion and migration assays in four glioma cell lines and patient-derived GBM cell lines after transfection with the pCMV3-SorCS3-Flag plasmid (Fig. 2A, C, F, I, Fig. S1B and Fig. S2A–C, Western blots were given in Original Data of Supplemental Material). This evidence concerns the gene SORCS3 and central nervous system cancer.