As HNSCCs are known the be subject to NK cell infiltration, an increased number of NK cells within the tumor correlates with better survival12, to further confirm the role of CHMP2A in regulating NK cell-mediated killing we did a similar CRISPR-Cas9 mediated knockout of CHMP2A in three different HNSCC cell lines (Fig. 2d and Supplemental Fig. 1b): Cal27 (tongue squamous cell carcinoma), Detroit 568 (metastatic pharyngeal carcinoma), and HNSCC17B (laryngeal squamous cell carcinoma)44. The gene discussed is CHMP2A; the disease is neoplasm.