Next, we detected the levels of PRC1 and NUF2 through RT-qPCR, western blotting and Immunohistochemical, and found that the expression of PRC1 and NUF2 in the tumor tissues of the siRNA-Gm31932-174 treated group was significantly reduced (Fig. 6H–J, Supplementary Fig. 7), which reconfirmed the results of in vitro experiments. This evidence concerns the gene NUF2 and neoplasm.