To confirm iPA action, GBM cell death was rescued through specific inhibitors of the proteins involved in the necroapoptotic pathway, NSA (MLKL inhibitor) and Nec-1 (RIP1 inhibitor); indeed the pretreatment with Nec-1 and NSA reduced the rate of necrotic cells detected by flow cytometry with PI staining. This evidence concerns the gene MLKL and glioblastoma.