PDCD1 and melanoma: Moreover, anti-VEGFR-1 mAb D16F7 enhanced the antitumor impacts of the anti-CTLA-4 and anti-PD-1 mAbs in B16F10 melanoma cell bearing mice most potently by augmented M1/M2 and CTLs/Tregs ratios, which offer an antitumor and immunostimulating TME [111].