Analyzing RNA-Seq data from Mischler et al. [62] (comparing the transcriptomes of TSC isolated from the human placenta with TSC differentiated from hESC) and Yabe et al. [63] (transcriptomes from syncytiotrophoblast generated from H1-ESC) showed that in contrast to colon and islets, the primary isoform of the NOS2 mRNA expressed was the isoform NOS2-2 (Fig. 4 and Additional file 1: Fig. S5). This evidence concerns the gene NOS2 and tuberous sclerosis.