The metabolic profiles of osteosarcoma cells were investigated by characterizing enzymes that play a role in glycolysis (PFK and LDHA), oxidative phosphorylation (ATPB), pentose-phosphate pathway (G6PDH), glutaminolysis (GLS), fatty acid beta oxidation (FASN) and long chain fatty acid synthesis (CPT1A). Here, GLS is linked to osteosarcoma.