ANGPTL4 and metastatic malignant neoplasm in the lymph nodes: To determine the predictive values of vascular invasion and lymph node metastasis in CCA patients, we analyzed ANGPTL4 levels and current tumor markers, CA 19-9 and CEA between patient groups by Mann-Whitney U-test and ROC analysis (Figures 4A,B, Table 3, Supplementary Figures 5A–F).