In this study, serum ANGPTL4 levels in CCA patients were found to be a better predictor of vascular invasion (cutoff = 0.5526 AU, sensitivity = 64.9%, specificity = 92.9%, AUC = 0.751) and lymph node metastasis (cutoff = 0.5399 AU, sensitivity = 71.4%, specificity = 70.8%, AUC = 0.691) than CA 19-9 and CEA. Here, ANGPTL4 is linked to cholangiocarcinoma.