We examined the total membrane CAT and P4HB protein expression and found a relatively higher expression of total CAT and P4HB proteins in the normal L02 liver cell line compared to the low metastatic MHCC-97L and high metastatic HCC-LM3 cell line (***p <0.001, Figures 4F–H). However, a progressively decreased expression of ECA-binding-galactosylated-CAT and P4HB was found in the high metastatic HCC-LM3 cell line compared to the low metastatic MHCC-97L and normal L02 liver cell line (***p <0.001, Figures 4F, I, J). This evidence concerns the gene CAT and hepatocellular carcinoma.