The previous WES studies from The Cancer Genome Atlas-Rectum adenocarcinoma (TCGA-READ) (49), after discarding the FLAGS genes TTN and MUC16, indicate the following top five most frequently mutated genes: APC (88.3%), TP53 (78.1%), KRAS (40.9%), FAT4 (21.2%), and FBXW7 (17.5%). Here, APC is linked to adenocarcinoma.