HES1 and Hyperglycemia: Concomitantly, EC treated with OICR-9429 and exposed to intermittent high glucose displayed lower levels of Jagged1 (Figure 4C), Jagged2 (Figure 4B), N1-ICD (Figure 4C), and Hes1 (Figure 4C) proteins compared with only intermittent high-glucose-challenged EC, indicating inhibition of Notch signaling activation in intermittent hyperglycemia-challenged HUVEC.