Latest studies showed that TFF1 can be used in the diagnosis and efficacy evaluation of breast cancer [25], and this study found that the sensitivity of TFF1 was 66.7%, which could be up to 95.0% when combining with CA15-3, CYFRA21-1, and MRI; its diagnostic accuracy rate was 87.2% and AUC (95%CI) = 0.892 (0.840–0.943), indicating that the diagnostic efficacy of combined diagnosis was significantly higher than that of single examination by CA15-3, CYFRA21-1, TFF1, or MRI. The gene discussed is TFF1; the disease is breast cancer.