MLKL and infection: At 72h post-infection, we found that the total protein of two major necroptosis biomarkers (MLKL and RIP3) and all three phosphorylated forms of necroptosis biomarkers (p-MLKL, p-RIP1 and p-RIP3) were elevated in L. interrogans infected splenocyte lysates (Figures 6A–G), though p-RIP3 and p-RIP1 were slightly increased in L. biflexa infected lysates, but p-MLKL was not increased (Figures 6E–G).