Cholestasis after ethinylestradiol is typically associated with ERa-JNK-c-JUN mediated (Ruiz et al., 2013) upregulation of Mrp3 basolateral efflux transporter for BAs (Hirsova et al., 2013; Muchova et al., 2015), which all were also reproduced in our study. Here, MAPK8 is linked to cholestasis.