To characterize the role of STAG2 in 3D genome organization in melanoma cells, we carried out ChIP-seq against STAG2, STAG1, SMC1A, and CTCF as well as Hi-C analyses (Supplementary Fig. 1a–c and Supplementary Data 1) in M14 melanoma cells stably expressing inducible shRNA of STAG26. The gene discussed is CTCF; the disease is melanoma.