The combination of heat shock protein 70 (HSP70) and sPD-1 vaccine increased the expression of the TH1 cytokines IL-2 and IFN-γ and decreased the expression of the negative regulatory molecules Foxp3, IL-10 and TGF-β in tumor-infiltrating lymphocytes of lung metastatic melanoma (61). The gene discussed is IFNG; the disease is metastatic melanoma.