In addition, the recombinant peptide sPD-1-CH50 was able to upregulate the expression of IFN-γ, tumor necrosis factor-α (TNF-α), and inducible nitric oxide synthase (iNOS) and to enhance the lytic activity of macrophages against B7-H1-positive tumor cells (60). This evidence concerns the gene CD274 and neoplasm.