Western blotting and qRT-PCR were performed and showed that treatment with SR717@RHE-HFn NPs upregulated the expression of STING downstream effectors and increased the mRNA levels of Ifnb1, Cxcl9, Cxcl10 and TNF-α, suggesting successful activation of the STING pathway by SR717 released under acidic conditions within glioma tissue. Here, IFNB1 is linked to glioma.