In respect to tumor aggressiveness, our results show that both PIP5K1αΔΝ and SG cells can trigger basement membrane disruption at the initial stage of infiltration, by using our newly developed in vivo chick based CAM-Delam method (Palaniappan et al., 2020), indicating that the N-terminal domain of PIP5K1α is not essential for PCa cells to disrupt the basal lamina at the initial stage of the growth and infiltration. Here, PIP5K1A is linked to neoplasm.