To clarify the mechanism of action of VPS72 in hepatocellular carcinoma, according to the ranking of VPS72 expression in hepatocellular carcinoma patients on The Cancer Genome Atlas (TCGA) database (https://portal.gdc.cancer.gov/), Gene Set Enrichment Analysis (GSEA) enrichment analysis revealed that VPS72 was mainly enriched in some specific signaling pathways. This evidence concerns the gene VPS72 and hepatocellular carcinoma.