For instance, MSI CRC was classified into two subtypes using a nonnegative matrix factorization algorithm.34 A subpopulation of pMMR/MSS patients showed increased CD8(+) TILs together with up-regulated IFN-γ via immunohistochemistry.35 A recent study further showed that MSS patients with or without chromosome 20q (Chr20q) amplification represented two subtypes of MSS CRC with distinct mutation profiles and immune cell infiltrations.36 However, there are still no studies to identify the molecular subtype of pMMR/MSS CRC. Here, CD8A is linked to colorectal carcinoma.