Further transcriptome analyses on public datasets (GSE3325, GSE32269 and GSE6919) showed that several key genes of the one-carbon metabolism pathway, including MTHFD2, MTHFD1, SHMT2, and SHMT1 were upregulated in CRPC tissues compared with primary localized PCa (Supplementary Fig. S4). Here, SHMT2 is linked to posterior cortical atrophy.