Flow cytometry analysis of microglial cells from these brains, analyzed after sequential gating (Fig. 1a), however, revealed an increase in the number of CD11b+CD45lo microglial cells gated in secondary metastatic tumor-bearing MMTV-Wnt1 mice, compared to non-tumor-bearing and primary tumor-bearing MMTV-Wnt1 mice (Fig. 1b). The gene discussed is WNT1; the disease is neoplasm.