CX3CL1 and migraine disorder: The experimental procedure is shown in Fig. 6A. Consistent with that of the comorbidity model rats, the mechanical pain threshold of the PBS-migraine group (0.94 ± 0.19) was decreased and was further decreased in the FKN-migraine group (0.12 ± 0.03) compared with the PBS-sham group (7.54 ± 0.96, Fig. 6B).