The protein levels of proBDNF/BDNF were also increased in the FKN-migraine group (6.44 ± 0.15; 1.87 ± 0.08) compared with the PBS-migraine group (3.92 ± 0.39; 1.64 ± 0.11, Fig. 6E-F). These results suggest that FKN can mimic the effect of epileptic seizures on migraine to some extent, suggesting that hyperexcited neurons after epileptic seizures may interact with microglia through the FKN/CX3CR1 axis to promote migraine. This evidence concerns the gene CX3CL1 and migraine disorder.