We performed a series of stratified grouping first divided into esophageal cancer patients, benign lesions and healthy people, always, our research has indicated that the signatures-associated antibodies related to the high-level expression may prompt early diagnosis of lung cancer, we applied to the determination of esophageal cancer patients, found that as shown, P53, PGP9.5, SOX2, CAGE, according to the results of quantitative of the signatures-associated antibodies in esophageal cancer-related levels also higher than that of benign disease and healthy people group. The gene discussed is SOX2; the disease is lung cancer.