Results are more credible in esophageal squamous carcinoma tissues, and grouping analysis showed SOX2 can be used as a kind of stem signatures-associated antibodies to detect early diagnosis of esophageal cancer occurrence, it may be through regulating NOTCH1, NOTCH2, ASCL4, FOXP1 pathways, influencing the tumour generation and progress [26–28]. This evidence concerns the gene ASCL4 and esophageal cancer.