We further analysed esophageal squamous cell carcinoma, benign lesions and healthy people and found that the AUC of each stem signatures-associated antibody was greater than 0.7, especially the SOX2 could reach 0.91 when his best cut-offs values were 4.55 U/ml for 91.7% and 85.4% of the diagnostic sensitivity and specificity for SOX2, which indicated a higher diagnostic efficiency. Here, SOX2 is linked to esophageal squamous cell carcinoma.