To further explore whether upregulation of IL-33 in stomach-cancer cells (MKN45 and BGC823) affects the secretion of IL-1β, IL-12, and IL-10 by the macrophages, ELISA was applied to detect the expressions of IL-1β, IL-12, and IL-10 in the supernatant after macrophages were stimulated with IL-33. The gene discussed is IL1B; the disease is gastric neoplasm.