The supernatants from the culture medium of cancer-cell groups (MKN45, BGC823), DLL3-overexpressed-cancer-cell groups (DLL3–MKN45, DLL3–BGC823), macrophage (Mφ) groups, macrophage-with-cancer-cell groups (Mφ + MKN45, Mφ + BGC823), and macrophage-with-DLL3-overexpressed-cancer-cell groups (Mφ + DLL3–MKN45, Mφ + DLL3–BGC823) were collected and the expression levels of IL-1β, IL-12, and IL-10 were detected using ELISA. Here, IL10 is linked to cancer.