At Stage I of tumor cell seeding, three media conditions were tested, NeuralBasal/B27 supplemented with fibroblast growth factor (FGF, 20 ng/mL) and epidermal growth factor (EGF, 20 ng/mL) as used for neural stem cells (“NB”), NeuralBasal/B27/EGF/FGF supplemented with 10% FBS (“N+FBS”), and “NB” media mixed at 1:1 ratio with VEGF-containing EGM-2 MV without serum (“NE”). This evidence concerns the gene EGF and neoplasm.