Tumor spheroids in 3D models contained significantly more Nestin+ (9.4 ± 1.2%) and CD133+ (putative cancer stem cell [18,38], 7.4 ± 1.1%) cells than 2D cultures (0.6 ± 0.4% and 2.4 ± 0.7%, respectively). This evidence concerns the gene NES and neoplasm.