To examine the mechanism of PRAME regulation by EZH2, we performed ChIP assays in EZH2-WT (DOHH2, HT, HBL-1, and SU-DHL-8) and EZH2-mut DLBCL cell lines (Karpas-422, SU-DHL-10, DB, WSU-DLCL2, SU-DHL-4, and SU-DHL-6) using anti-H3K27me3 antibody. The gene discussed is EZH2; the disease is diffuse large B-cell lymphoma.