PRAME and diffuse large B-cell lymphoma: In Karpas-422, the CD8+CD69+ population was not significantly different between PRAME-WT and -KO groups, although we observed a significant difference in the activation status of cocultured naive CD4+ T cells (CD4+CD69+ population; Figure 2E and Supplemental Figure 6B), matching the findings in the primary DLBCL samples (Figure 2C).