In particular, we focus on PLK1, Aurora kinases, Rho-associated protein kinase 1 (ROCK1), and integrin-linked kinase (ILK) because these mitotic kinases regulate mitotic dynamics and metastasis via posttranslational modification of several substrates, including cytoskeletal proteins, in a manner dependent on extracellular signaling and the tumor microenvironment, as discussed below. The gene discussed is PLK1; the disease is neoplasm.