TNF and colorectal carcinoma: The second set of samples comprised 9 CRA, 9 CIA, and 9 CRC, with IHC staining for macrophages (CD68), M1 macrophages (iNOS) and M2 macrophages (CD163), and RNA ISH for expression of immunosuppressive cytokines IL-10 and TGF-β, and inflammatory cytokines TNF-α and IL-6.