Further, the abnormal expressions of ITGα1, ITGα3, ITGα5, ITGα6, ITGα8, ITGα9, ITGα11 and ITGβ3 were verified by qRT-PCR and western blot, indicating these integrins may be key regulators in tumor formation and metastasis processes induced by co-infection of REV and ALV-J. This evidence concerns the gene ITGA5 and coinfection.