This study verified the expression of 10 candidate differential genes screened from cervical microbes related to CIN progression pathways in different cervical tissues through fluorescence quantitative PCR: ATM, ABCG2, PCNA, OGG1, TDG, LIG1, and HMGB1. The gene discussed is HMGB1; the disease is cervical squamous intraepithelial neoplasia.