In the present study, we found that the protein levels of phosphorylated ERK-1/2 and the ratio of p-ERK-1/2/ERK-1/2 were both significantly decreased after ThPOK overexpression or STPG1 upregulation in gastric cancer cells, which indicated that ThPOK and STPG1 exerted inhibitory effects on the ERK signaling pathway. Here, STPG1 is linked to gastric cancer.