AFP and hepatocellular carcinoma: Alpha-fetoprotein (AFP) was once the most classic marker for the diagnosis of HCC, but about 20%–30% of HCC patients had negative or low AFP expression [5]; for imaging screening, color Doppler ultrasound is currently the most widely used imaging screening tool because of its noninvasive and convenient nature, but ultrasonography may be affected by rib obscuration, abdominal wall, intestinal gas, and other factors that may lead to misdiagnosis imaging [6].