In our study, knockdown of SPRY4-IT1 decreased the protein expression levels of Snail, Twist1 and Vimentin, and increased the protein expression level of E-cadherin, which suggested that knockdown of SPRY4-IT1 inhibited NPC cell metastasis might via the regulation of EMT pathway. The gene discussed is SNAI1; the disease is nasopharyngeal carcinoma.