PRRX1 and atrial fibrillation: Sanger sequencing analysis in the family revealed that only the variant chr1:170,688,998G>T (GRCh37/hg19: NC_000001.10), equivalent to chr1:170,719,857G>T (GRCh38/hg38: NC_000001.11) or NM_022716.4:c.373G>T;p.(Glu125*) in the PRRX1 gene, was in co-segregation with AF and PDA in the whole family, with complete penetrance.