To verify whether there is concurrent enrichment with convergent TCR clusters, we performed fluorescence-activated cell sorting (FACS) of CD39+PD-1+ (double-positive [DP]) and non-DP CD4+ and CD8+ T cells from TILs freshly isolated from lymph node metastases from eight melanoma patients (Figure 2a, Figure 2—figure supplement 1a). This evidence concerns the gene CD4 and melanoma.